International Union for Circumpolar Health Ministry of Public Health and Social Development of RF Russian Academy of Medical Sciences Siberian Branch of Russian Academy of Medical Sciences Siberian Branch of Russian Academy of Sciences Medical Polar Fund “Science” The Northern Forum

Abstracts

Ethnic peculiarities of the North native inhabitants health

Aim. To study Helicobactеr Pilory (НР), genotypes, the relationship between proliferation and epitheliocyte apoptosis in ulcer disease of duodenum in the Europoid and Mongoloid of Khakassia.

Method. We examined 27 Europoid and 24 Khakass, duodenum ulcer disease patients. Immune gistochemical test was carried out in bioptats from three parts of stomach, using streptavidin-biotin method. As initial antibodies we used mice monoclonal antibodies: proliferation antigen Кi-67, representative of caspase effector СРР-32, which regulates apoptosis irreversible stage. We determined proliferation index as % share of positively colored epitheliocytes. In all the patients Helicobacter Pylori infection was found by morphological and urease methods. Besides we determined Vac A, Cag A, Ice A, Bab A types and subtypes of НР by microbiological and polymer-chain-reaction (PCR) methods.

Results. Cag A and S1S2 subtypes of HP Vac A strains were registered in 93.3% and 70.0% Europoid and in 57.1% (P<0.01) and 42.9% (P<0.05) Mongoloid. Proliferation index (PI) in the Khakass was evidently higher, than in Europoid. Apoptosis index (AI) in ulcer diseases was 32.0% in the Khakass and 32.9% in the Europoid and was higher (P<0.01), than in patients without ulcer disease. PI/ AI coefficient in both target populations in ulcer disease was lower, than 1 (0.71 in the Khakass; 0.62 in the Europoid), which is caused by apoptosis hyperfunction.

Conclusion. There are definite differences in proportions between proliferation and apoptosis processes in the examined target population, which can be connected with differences between HP genotypes

Note. Abstracts are published in author's edition

© 1996-2005, Siberian Branch of Russian Academy of Sciences, Novosibirsk
Last update: 06-Jul-2012 (11:52:05)