International Union for Circumpolar Health Ministry of Public Health and Social Development of RF Russian Academy of Medical Sciences Siberian Branch of Russian Academy of Medical Sciences Siberian Branch of Russian Academy of Sciences Medical Polar Fund “Science” The Northern Forum

Abstracts

Ecology and adaptation (medical ecology)

The main parameters of red blood and enzymatic status of rats subjected to forced physical load and cooling with different diet rations at the background have been analyzed.

The experiments were conducted with white male rats.The rats have been divided into 3 groups: one reference group and 2 experimental groups. As a reference, there were used intact rats that were at normal diet of the vivarium.

The experimental rats were grouped in the following way: in the first experimental group, physical load was combined with cooling at the background of normal diet, in the second group – at the background of enriched diet. Forced physical load consisted of placement into a rotating wheel for 10 minutes every day during 30 days. When rats were cooled, they were placed into a cooling chamber by the temperature +5°C. In the second group, the diet was enriched with fluorine and iron compounds. Fluorine was prescribed in the form of sodium fluoride with kissel or milk every second day (fluorine dose = 0.1 mg/kg of weight). Hemoiron was prescribed in the dose 0.17 mg/kg.

The animals were taken out of the experiment (30-th day) by way of decapitation according to a method merciful for the rats. The hematologic indices of blood [content of erythrocytes; hemoglobin concentration in blood; the average volume of erythrocytes; the average content of hemoglobin in an erythrocyte (AContHE); the average concentration of hemoglobin in an erythrocyte (AConcHE)], alkaline phosphatase and alanine aminotranspherase were determined according to the generally used methods. The statistic working was done according to the method of Student.

The analysis of red blood indices in the rats from the first experimental group has revealed a reliable decrease in the content of erythrocytes and hemoglobin concentration in blood in comparison with the reference group. The study of the erythrocyte markers has revealed a multi-vector tendency of indices’ change. In particular, the average volume of erythrocytes increased reliably by unchanged AcontHE what caused a reliable decrease of AConcHE . Such changes are unfavorable both for the structure of erythrocyte membrane and for its transport function’s implementation. A decrease in activity of alkaline phosphatase and alanine aminotranspherase correspondingly by 17.5 and 13.1% was observed.

In the second experimental group, enriched diet with introduction of food containing fluorine and iron compounds influenced red blood indices in the following way: there was observed an increase in the number of erythrocytes both in comparison with the group subjected to unfavorable conditions and that did not get additional diet and in comparison with the reference group .Hemoglobin concentration in blood was restored up to the indices of the reference group.

A reliable increase in activity of enzymes of alkaline phosphatase and alanine aminotranspherase was observed, what was also indicative of favorable influence of enriched diet on the experimental animals’ organisms, in particular on the liver by influence of unfavorable environmental factors.

Note. Abstracts are published in author's edition

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Last update: 06-Jul-2012 (11:52:05)