Информационная система "Конференции"

Abstracts

APPLICATION OF COMPUTER TECHNOLOGIES TO ANALYSIS OF MICROSCOPIC OBJECTS FROM LAKE BAIKAL

O.V. Kotsar, T.A. Khodzher, V.N. Drozdov, Yu.P. Sapozhnikova
Limnological Institute SB RAS, 3, Ulan-Batorskaya, Irkutsk 664033, Russia, E-mail: jonin@lin.irk.ru

The scientists of Limnological Institute of the Siberian Branch of the Russian Academy of Sciences have been applied recent technologies to studying biodiversity and evolution of endemic Baikal organisms, as well as to searching optimal methods of their 3D modelling.

Calculation of diatoms identified with Programme Image-Pro. It was possible to reconstruct paleoclimates of the sedimentary thickness of Lake Baikal due to results of diatom studies as diatoms are one of the most sensitive markers of glacials and interglacials (Grachev et al., 1997). Programme Image-Pro allows to separate contact valves of diatoms with different variations of light intensity, contrast and gamma-correction of the initial image of diatoms. However, using such manipulations the image completely changes in some parts. It causes large errors in counting. Moreover, everything is done manually and therefore it is a labour process. The problem of division of contact overlaying valves remains unsolved, as well as an effect on the image of microscope focus is observed. To solve this problem we applied an anaglyph method when a specialist identify diatoms visually on green-red stereo images which makes the work of a researcher easier. The image prepared in this way allows to see a pseudo-volume and estimate the appropriateness of this stereo pair to reconstruction of the object surface with the help of photogrammetry.

The study of geometric characteristics of biological microorganisms is an actual recent problem for solution of a number of environmental problems. The reconstruction of the object surface of the real microworld may be achieved with different methods the most perspective of which are photogrammetric methods suggesting the so-called a non-contact and "non-destructive" reconstruction.

The creation of a model of rotifer Keratella cochlearis cochlearis was an approbation of modelling of microscopic live organisms from Lake Baikal. This biological object has been chosen as an example for modelling due to its short life cycle and extreme sensitivity to changes in the environment. The solid model of rotifer was compiled with software of 3D modelling. Only the first step in modelling of Baikal invertebrate has been done which opens perspectives in further studies.

It is planned to test a technique of reconstruction of microorganisms using GIS and sophisticated software Programme of Analysis 3D Model (PA-3DM) for studying stereo images and compiling solid models with non-contact technique which allows to perform geometric measurements, analysis of morphology, species division of organisms of one species and to carry out monitoring of the environment.

This work was supported by Presidium Programme of the Russian Academy of Sciences 18.10.

References

1. Pernthaler J., Pernthaler A., Amann R. Automated enumeration of groups of marine picoplankton after fluorescence in situ hybridization. // Appl. Environ. Microbiol. 2003. V. 69. P. 2631-2637.
2. Drozdov V.I., Sergeeva V.I., Maksimenko S.Yu., Zemskaya T.I. Computer system on recording of images of fluorescence stained bacteria // Microbiology, 2006, vol. 75, No. 5, p. 1-4.
3. Khodzher T.A., Bychkov I.V., Tyagun M.L. Application of digital photogrammetry to reconstruction of relief of Baikal omul scales // Computational Technologies, 2005, vol. 10, No. 4, p. 107-110

Note. Abstracts are published in author's edition

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