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Θνρςθςσς φθςξλξγθθ θ γενεςθκθ

First Workshop on Information Technologies Application to Problems of Biodiversity and Dynamics of Ecosystems in North Eurasia (WITA-2001)

July 9-14, 2001, Novosibirsk, Russia

Abstracts

Genetic Bases of Biodiversity

Comparative analysis of the nucleotide composition in mitochondrial genomes

Ivashchenko A.T., Goncharova A.V., Ivashchenko T.A

Kazakh State National University (Almaty)

Drastic increase of the completely sequenced mitochondrial genomes number gives the opportunity for computational comparative analysis of mitochondrial DNA. We found that in the single strand of mitochondrial DNA nucleotide frequencies (fA,fG,fT,fC) obey the rule called by us as “four-nucleotide-rule” (FNR), saying that the difference between the complementary nucleotide rations are proportional to purine content (fR) in a given DNA fragment: fA/fT-fC/fG = a(fR-0,5)+b, where a and b are the linear regression parameters. Calculated regression parameters reflecting FNR for single mitochondrial DNA strand from over 50 species, shows substantial diversity and for some of the species the data are listed below:

Species

Taxon

Regression

r ±Sr

Acanthamoeba castelliana

Acanthamoebidae

δελόςΰ=6,06(fR- 0,5)+0,12

0,94±0,03

Arabidopsis thaliana

Magnoliophyta

δελόςΰ=8,04(fR- 0,5)- 0,01

0,99±0,01

Asterina pectinifera

Echinodermata

δελόςΰ=12,3(fR- 0,5)- 0,26

0,86±0,07

Chondrus crispus

Phodophyta

δελόςΰ=7,17(fR- 0,5)+0,22

0,92±0,04

Caenorabditis elegans

Nematoda

δελόςΰ=4,43(fR- 0,5)+0,27

0,85±0,08

Chlamydomonas reinhardtii

Chlorophyta

δελόςΰ=7,59(fR- 0,5)+0,10

0,97±0,04

Echinococcus multilocularis

Platyhelmintes

δελόςΰ=3,10(fR- 0,5)+0,29

0,86±0,08

Homo sapiens

Chordata

δελόςΰ=20,0(fR- 0,5)- 0,21

0,78±0,09

Hansenula wingei

Fungi

δελόςΰ=7,32(fR- 0,5)+0,11

0,93±0,04

Katharina tunicata

Mollusca

δελόςΰ=5,87(fR- 0,5)+0,18

0,90±0,06

Locusta migratoria

Arthropoda

δελόςΰ=8,89(fR- 0,5)- 0,42

0,80±0,09

Lumbricu terrestis

Annelida

δελόςΰ=10,5(fR- 0,5)- 0,08

0,88±0,07

Marchancia polymorpha

Embryophyta

δελόςΰ=7,89(fR- 0,5)+0,01

0,98±0,03

Physarum polycephalum

Myxogastria

δελόςΰ=7,11(fR- 0,5)- 0,01

0,90±0,03

Tetrahymena pyroformis

Alveolata

δελόςΰ=7,18(fR- 0,5)+0,01

0,72±0,06

where Δ=fA/fT-fC/fG, and r±Sr - correlation coefficient with the standard error. Data were obtained by analyzing the stretches of single DNA strands with the length of 300 nucleotides. Substantial heterogeneity of structural-functional properties of mitochondrial DNA reflects in large variability of FNR regression parameters. Nucleotide composition of the different functional stretches of single strand of DNA (protein coding genes, tRNA, rRNA, introns, intergenic spacers) obey the FNR for a given genome. Various aspects of FNR application for comparative and evolutionary analysis of mitochondrial DNA and their functional regions are discussed.

Note. Abstracts are published in author's edition

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