Abstracts

Genetic Bases of Biodiversity

It is now well established that chromosome painting is an economical and efficient method to map chromosomal homology between species. Chromosome painting has been valuable in identifying ancient syntenies shared by widely divergent species. These syntenies indicate the likely struc-ture of ancestral chromosomes lit can usually be assumed that when large blocks of homologous DNA are retained intact in distantly related species (outgroups), this arrangement represents the ancestral condition. Hybridisation of human chromosome-specific paints to a wide range of mammalian species has revealed syntenic blocks which are separated on dif-ferent human chromosomes but contained in one syntenic block on a single chromosome in a number of outgroup species, indicating that the arrangement in the outgroup species is ancestral to them all.

Previously obtained and new generate chromosome specific probes for mammalian species from different taxa (human, mink, fox, cat, dog, Chinese hamster) from Centre for Veterinary Science, Department of Clinical Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB3 OES, UK had been used for produce detail comparative maps for the large number species, including most part of domestic, domesticated and laboratory species. Mapping the same probes onto DAPI and high resolution G-banded chromosomes allow us to analyze all details of karyotype and genome evolution in Mammalia and produce complete integrative maps for human and mammals.

Reference
Graphodatsky AS, Yang F, O'Brien PCM, Serdukova N, Milne BS, Trifonov V, Ferguson-Smith MA (2000) A comparative chromosome map of the Arctic fox, red fox and dog defined by chromosome painting and G-banding. Chromosome Res, 8: 253 - 263
Graphodatsky AS, Yang F, Serdukova N, Perelman P, Zhdanova N, Ferguson-Smith MA (2000) Dog chromosome-specific paints reveal evolutionary inter- and intrachromosomal rearrangements in the American mink and human Cytogenet Cell Genet. 90: 275-278.
Rens W., P. C. M. O’Brien, F. Yang, N. Solanky, P. Perelman, A. S. Graphodatsky, M. W. J. Ferguson, M. Svartman, A. A. De Leo, J. A. M. Graves, M. A. Ferguson-Smith (2001) Karyotype relationships between distantly related marsupials from South America and Australia ChromosomeRes., 9: 301-308
Yang F, O'Brien PCM, Milne BS, Graphodatsky AS, Solanky N, Trifonov V, Rens W, Sragan D, Ferguson-Smith MA (1999) A complete comparative chromosome map for the dog, red fox and human and its integration with canine genetic maps. Genomics 62: 189-202
Yang Fengtang, Alexander S. Graphodatsky, Patricia C. M. O’Brien, Amanda Colabella, Nita Solanky, Michael Squire, David R. Sargan, Malcolm A. Ferguson-Smith (2000) Reciprocal chromosome painting illuminates the history of genome evolution of the domestic cat, dog and human. Chromosome Res, 8: 393-404
AS Graphodatsky (2001). Chromosome painting in comparative cytogenetics. Membrane and Cell Biol., 18: 173-179
AS Graphodatsky , F Yang , PCM O'Brien , P Perelman, BS Milne , N Serdukova, S-I Kawada and MA Ferguson-Smith (2001) Chromosomal segments ancestral for the Canidae inferred from comparative chromosome painting. Cytogenet Cell Genet 92: (in press)

Note. Abstracts are published in author's edition

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