Информационная система "Конференции"

Всероссийский конгресс «Нефрология и диализ сегодня».

Россия, Новосибирск, с 15 по 17 сентября 2003 г.

Тезисы докладов

Novel Role for Renal Na,K-ATPase as a Gene-regulator

Aizman O.R., Uhlen P. & Anita Aperia

Karolinska Institutet,
Astrid Lindgren`s Children Hospital,
Dept. of Woman and Child Health (Stockholm)

Recent studies have indicated that Na,K-ATPase may, in addition to being the key regulator of intracellular Na+ and K+ concentration, act as a signal transducer. The biological role for ouabain, the natural ligand of Na,K-ATPase, has, despite extensive research, not been well understood. We have reported that exposure of rat proximal tubular cells (RPTC), to doses of ouabain that inhibit the Na,K-ATPase activity less than 50 % (10 nM – 500 µM), will induce intracellular [Ca2+]i oscillations and that this calcium signal leads to activation of the transcription factors NF-kB. The ouabain induced calcium oscillations were blocked by an inhibitor of the IP3 receptors but not by phophospholipase C inhibitors nor by cellular depletion of IP3, suggesting that the calcium signal is not due to phospholipase C mediated IP3 release. Fluorescence resonance energy transfer (FRET) studies suggested a close proximity between the Na,K-ATPase and IP3 receptor. Our findings demonstrate a novel principle for calcium signaling via Na,K-ATPase. Thus we present a novel principle for a cell signaling microdomain consisist of the Na,K-ATPase and IP3 receptor, where an ionic pump serves as a receptor and signal transducer.

Примечание. Тезисы докладов публикуются в авторской редакции

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